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Photosystem I–hydrogenase fusion in cyanobacteria

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The Photosystem I–hydrogenase fusion is a genetic engineering strategy in which a hydrogenase enzyme is covalently linked to a component of Photosystem I, enabling direct transfer of photosynthetic electrons to hydrogenase and thereby enhancing light‑driven hydrogen production.[1][2] In the reported system, cyanobacterial mutants expressing such a fusion showed significantly longer and more stable hydrogen production compared with wild‑type cells when immobilized in the protective redox polymer matrix.[1][2]

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A biohybrid electrode featuring viologen-shielded cyanobacteria expressing a Photosystem I–hydrogenase fusion delivers continuous hydrogen production under ambient oxygen, marking a key step in bio-photovoltaic green hydrogen.

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